Module:Bio::Tools::Run::Primer3

This module is a wrapper for Primer3.

How do I set a parameter when running this module?

Set the command line parameters by just calling a function of the name of the parameter. To get a list of the available options, this perl code will report it to you:

# what are the arguments, and what do they mean?
my $args = $primer3->arguments;
 
print "ARGUMENT\tMEANING\n";
foreach my $key (keys %{$args}) {print "$key\t", $$args{$key}, "\n"}

The info for PRODUCT_SIZE_RANGE is:

(size range list, default 100-300) space separated list of product sizes eg <a>- <x>-<y>

I believe you can set the PCR product size with

$primer3->add_target(PRIMER_PRODUCT_SIZE_RANGE => "490-510");

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Pdoc documentation: Bio::Tools::Run::Primer3	CPAN documentation: Bio::Tools::Run::Primer3

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