# $Id: INSTALL 15578 2009-02-26 02:17:03Z bosborne $ bioperl-run INSTALLATION INSTALL THE RIGHT BIOPERL You need at least the corresponding version of Bioperl. Since this is BioPerl-run 1.6.1, you should use BioPerl 1.6. INSTALLATION Installation instructions at the following address apply here: http://www.bioperl.org/wiki/Installing_Bioperl_for_Unix The next 2 sections summarize the essential points from there. CPAN INSTALLATION To install using CPAN you will need a recent version (v1.8802 has been tested) of it and your prefer_installer conf set to 'MB': >cpan cpan>o conf prefer_installer MB cpan>o conf commit cpan>q Find the name of the bioperl-run version you want: >cpan cpan>d /bioperl-run/ Database was generated on Mon, 20 Nov 2006 05:24:36 GMT Distribution C/CJ/CJFIELDS/BioPerl-run-1.6.1.tar.gz Now install: cpan>install C/CJ/CJFIELDS/BioPerl-run-1.6.1.tar.gz If you've installed everything perfectly then you may pass all the tests run in the './Build test' phase. It's also possible that you may fail some tests. Possible explanations: problems with local Perl installation, previously undetected bug in Bioperl, flawed test script and so on. A few failed tests may not affect your usage of bioperl-run. If you decide that the failed tests will not affect how you intend to use bioperl-run and you'd like to install anyway do: cpan>force install C/CJ/CJFIELDS/BioPerl-run-1.6.1.tar.gz This is what most experienced Bioperl users would do. However, if you're concerned about a failed test and need assistance or advice then contact bioperl-l@bioperl.org. MANUAL INSTALLATION Download the bioperl-run archive, then extract its contents. Example: >gunzip bioperl-run-.tar.gz >tar xvf bioperl-run-.tar >cd bioperl-run where is the current release. Issue the following command from within bioperl-run/: >perl Build.PL You can run regression tests and install bioperl-run using the following commands: >./Build test >./Build install NOTE: many tests will be skipped without the necessary environment variables set to tell Bioperl where your programs are installed. INSTALLING bioperl-run ON WINDOWS The following page on the BioPerl website has up-to-date instructions on how to install bioperl-run on Windows: http://www.bioperl.org/wiki/Installing_Bioperl_on_Windows (the instructions are aimed at bioperl-core, but apply equally to bioperl-run) ENVIRONMENT VARIABLES Some important environment variables you need to be aware of. Variable Values Comment -------------------------------------------------------------------- PHYLIPVERSION 3.5, 3.6 If you want to run Phylip3.6 you need to set this env variable to 3.6 BLASTDIR DIR PATH Point to the directory where BLAST is installed GENSCAN_DIR DIR PATH Point to the directory where HumanIso.smat file is installed EPONINEDIR DIR PATH Point to the directory where eponine_scan.jar is installed PAMLDIR DIR PATH Point to directory where PAML is installed Generally the all-caps program name concatenated to 'DIR' patten for environment variable names is followed for most programs. However there are some exceptions (some require an underscore between the program name and 'DIR'), so check the documentation for the module you're interested in using. Note that for some programs, having the executables in your path is not enough - the correct environment variable still needs to be set, and sometimes it shouldn't point to the executable location, but somewhere else - again, check the documentation.